Thi-Mai studied classical and contemporary dance at the CNSM in Paris from 1995 to 1999 where she approached different repertoires such as Angelin Preljocaj, Jean Claude Brumachon, Rui Horta. She studied at P.A.R.T.S for a year and a half then joined the choreographer Wim Vandekeybus at Ultima Vez from 2002 to 2012. She has performed in Blush, Puur, Spiegel, Radical Wrong, Oedipe and in the films Blush and HERE AFTER. She works with Michèle Anne De Mey on the revival of the piece Sinfonia Eroïca.
In 2012, she joined the director James Thiérrée for Tabac Rouge and La Grenouille avait raison. Thi-Mai assists James Thiérrée at the choreography for his project Frôlons at the Opéra Garnier in May 2018, for 45 dancers, then on his new creation ROOM. She worked as a choreographer on the movie La Vérité by Hirokazu Kore-Eda.
On October 1st 2018, ETNA, Thi-Mai Nguyen's first solo received the Critics Prize for the best dance show in Belgium. Thi-Mai premiered her second solo PREMISSE at the Théâtre Marni on May 12th 2021. Her new creation STAND BY played from March 8th to 12th 2022 at the Théâtre la Balsamine in Brussels.